NSE Antibody
Rabbit mAb
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, FC, ICC |
---|---|
Primary Accession | P09104 |
Reactivity | Rat |
Clonality | Monoclonal |
Other Names | ENO2; ENOG; Enolase 2; enolase 2 (gamma, neuronal); Gamma-enolase; Neural enolase; neuron specific gamma enolase; Neuron-specific enolase; neurone-specific enolase; NSE; |
Isotype | Rabbit IgG |
Host | Rabbit |
Calculated MW | 47269 Da |
Dilution | WB 1:5000~1:10000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:50 |
---|---|
Purification | Affinity-chromatography |
Immunogen | A synthesized peptide derived from human NSE |
Description | ENO2 an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. |
Storage Condition and Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle. |
Name | ENO2 |
---|---|
Function | Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium- dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity). |
Cellular Location | Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form |
Tissue Location | The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons |
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