CHPF Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC, IHC-P, WB, E |
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Primary Accession | Q8IZ52 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 85467 Da |
Antigen Region | 327-354 aa |
Gene ID | 79586 |
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Other Names | Chondroitin sulfate synthase 2, Chondroitin glucuronyltransferase 2, Chondroitin-polymerizing factor, ChPF, Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II, N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase II, N-acetylgalactosaminyltransferase 2, CHPF, CSS2 |
Target/Specificity | This CHPF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 327-354 amino acids from the Central region of human CHPF. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | CHPF Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CHPF (HGNC:24291) |
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Synonyms | CSS2 |
Function | Has both beta-1,3-glucuronic acid and beta-1,4-N- acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP- GalNAc to the non-reducing end of the elongating chondroitin polymer. Seems to act as a specific activating factor for CHSY1 in chondroitin polymerization (PubMed:12716890). |
Cellular Location | [Isoform 1]: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Cytoplasm, cytosol [Isoform 2]: Mitochondrion matrix |
Tissue Location | Ubiquitous. Highly expressed in pancreas, ovary, brain, heart, skeletal muscle, colon, kidney, liver, stomach, spleen and placenta. [Isoform 3]: Also ubiquitous. |
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Provided below are standard protocols that you may find useful for product applications.
Background
CHPF is a protein that has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer.
References
Matsuoka,S., et.al., Science 316 (5828), 1160-1166 (2007)
Colland,F., et.al., Genome Res. 14 (7), 1324-1332 (2004)
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