nNOS (neuronal) Antibody
Rabbit mAb
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, FC, ICC, IP |
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Primary Accession | P29475 |
Reactivity | Rat |
Clonality | Monoclonal |
Other Names | bNOS; Constitutive NOS; IHPS1; N-NOS; NC-NOS; Neuronal NOS; nitric oxide synthase 1 (neuronal); Nitric oxide synthase, brain; nNOS; NOS; NOS type I; NOS1; |
Isotype | Rabbit IgG |
Host | Rabbit |
Calculated MW | 160970 Da |
Dilution | WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50 |
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Purification | Affinity-chromatography |
Immunogen | A synthesized peptide derived from human nNOS (neuronal) |
Description | nNOS nitric oxide synthase 1, neuronal form. Produces nitric oxide which is a messenger molecule with diverse functions and displays many properties of a neurotransmitter in the brain and peripheral nervous system. May be an effector enzyme for the dystrophin complex. |
Storage Condition and Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle. |
Name | NOS1 (HGNC:7872) |
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Function | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. |
Cellular Location | Cell membrane, sarcolemma {ECO:0000250|UniProtKB:Q9Z0J4}; Peripheral membrane protein. Cell projection, dendritic spine {ECO:0000250|UniProtKB:P29476}. Note=In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex (By similarity) In neurons, enriched in dendritic spines (By similarity) {ECO:0000250|UniProtKB:P29476, ECO:0000250|UniProtKB:Q9Z0J4} |
Tissue Location | Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland |
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