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Rad51 Antibody
Rabbit mAb
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC, IP |
|---|---|
| Primary Accession | Q06609 |
| Reactivity | Rat, Human, Mouse |
| Clonality | Monoclonal |
| Other Names | RAD51; BRCC5; MRMV2; RECA; DNA repair protein rhp51;RAD51; BRCC5; MRMV2; RECA; DNA repair protein rhp51; |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Calculated MW | 36966 Da |
| Dilution | WB 1:500~1:2000 IP 1:50 FC 1:50 |
|---|---|
| Purification | Affinity-chromatography |
| Immunogen | A synthesized peptide derived from human Rad51 |
| Description | Rad51 is a recombinase that is essential for homologous recombination and double-strand break repair. It binds to single and double-stranded DNA, and has DNA-dependent ATPase activity. Rad51 unwinds duplex DNA and forms helical nucleoprotein filaments in a manner that is controlled through the action of DNA helicases that counteract nucleofilament formation. Rad51 interacts with BRCA1 and BRCA2, and also plays a role in p53-mediated signalling. Interaction of Rad51 with Chk1 kinase may occur in response to Chk1 phosphorylation. |
| Storage Condition and Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle. |
| Name | RAD51 (HGNC:9817) |
|---|---|
| Synonyms | RAD51A, RECA |
| Function | Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR) (PubMed:12205100, PubMed:18417535, PubMed:20231364, PubMed:20348101, PubMed:22325354, PubMed:23509288, PubMed:23754376, PubMed:26681308, PubMed:28575658, PubMed:32640219). Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:12205100, PubMed:18417535, PubMed:20231364, PubMed:20348101, PubMed:23509288, PubMed:23754376, PubMed:26681308, PubMed:28575658). Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template (PubMed:12205100, PubMed:18417535, PubMed:20231364, PubMed:20348101, PubMed:23509288, PubMed:23754376, PubMed:26681308, PubMed:28575658, PubMed:38459011). Recruited to resolve stalled replication forks during replication stress (PubMed:27797818, PubMed:31844045). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR (PubMed:12442171, PubMed:24141787). Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3 (PubMed:20413593). Also involved in interstrand cross-link repair (PubMed:26253028). |
| Cellular Location | Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix Chromosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage (PubMed:20154705). DNA damage induces an increase in nuclear levels (PubMed:20154705). Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment (PubMed:23754376). Accumulated at sites of DNA damage in a SPIDR- dependent manner (PubMed:23509288). Recruited at sites of DNA damage in a MCM9-MCM8-dependent manner (PubMed:23401855). Recruited at sites of DNA damage following interaction with TOPBP1 in S-phase (PubMed:26811421). Colocalizes with ERCC5/XPG to nuclear foci in S phase (PubMed:26833090). Recruited to stalled replication forks during replication stress by the TONSL-MMS22L complex, as well as ATAD5 and WDR48 in an ATR-dependent manner (PubMed:27797818, PubMed:31844045) |
| Tissue Location | Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast |
Citations (0)
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Application Protocols
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$ 350.00
$ 175.00
Cat# AP90337
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