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TBB1 Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - TBB1 Antibody AP9023A
    All lanes : Anti-TBB1 Antibody at 1:2000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - TBB1 Antibody AP9023A
    Formalin-fixed and paraffin-embedded human brain tissue reacted with TBB1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - TBB1 Antibody AP9023A
    TBB1 Antibody (Cat. #AP9023a) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q9H4B7
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 50327 Da
Additional Information
Gene ID 81027
Other Names Tubulin beta-1 chain, TUBB1
Target/Specificity This TBB1 antibody is generated from rabbits immunized with human TBB1 recombinant protein.
Dilution WB~~1:2000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTBB1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TUBB1
Function Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Cellular Location Cytoplasm, cytoskeleton
Tissue Location Hematopoietic cell-specific. Major isotype in leukocytes, where it represents 50% of all beta-tubulins
Research Areas
Citations (0)
citation

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Background

The tubulin family of globular proteins has several members, the most common of which are a-tubulin and ?tubulin; proteins which make up microtubules of the cytoskeltons of probably all eukaryotic cells. Except in the simplest eukaryotes, tubulin (100 kDa) exists in all cells as a heterodimer of two similar but non-identical polypeptides (55 kDa each), designated alpha and beta. Within either family of alpha/beta tubulin heterodimers, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the carboxyl-terminal 15 residues. Delta (d) and epsilon (e) tubulin have been found to localize at centrioles and may play a role in forming the mitotic spindle during mitosis, though neither is as well-studied as the a- and ? forms.

References

Rogowski K., et.al., Cell 137:1076-1087(2009).

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$ 365.00
$ 140.00
Cat# AP9023A
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