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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CLGN Antibody (Center)   

CLGN Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CLGN Antibody (Center) AP9022c
    Western blot analysis of CLGN Antibody (Center) (Cat. #AP9022c) in 293 cell line lysates (35ug/lane). CLGN (arrow) was detected using the purified Pab.
    detail
  • IHC-P - CLGN Antibody (Center) AP9022c
    Formalin-fixed and paraffin-embedded human testis tissue reacted with CLGN Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - CLGN Antibody (Center) AP9022c
    CLGN Antibody (Center) (Cat. #AP9022c) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession O14967
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 70039 Da
Antigen Region 309-336 aa
Additional Information
Gene ID 1047
Other Names Calmegin, CLGN
Target/Specificity This CLGN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-336 amino acids from the Central region of human CLGN.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCLGN Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CLGN
Function Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions (By similarity).
Cellular Location Endoplasmic reticulum membrane; Single-pass type I membrane protein
Tissue Location Detected in testis (at protein level). Detected in testis.
Research Areas
Citations ( 0 )

Background

CLGN probably plays an important role in spermatogenesis. Binds calcium ions.

References

van Lith M., et.al., Mol. Biol. Cell 18:2795-2804(2007).

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$ 365.00
$ 140.00
Cat# AP9022c
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