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ETV6 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - ETV6 Antibody (N-term) AP8917a
    All lanes : Anti-ETV6 Antibody (N-term) at 1:2000 dilution Lane 1: U266B1 whole cell lysate Lane 2: U-2OS whole cell lysate Lane 3: Raji whole cell lysate Lane 4: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 53 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - ETV6 Antibody (N-term) AP8917a
    Formalin-fixed and paraffin-embedded human testis tissue reacted with ETV6 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - ETV6 Antibody (N-term) AP8917a
    ETV6 Antibody (N-term) (Cat. #AP8917a) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession P41212
Other Accession P97360, Q0VC65
Reactivity Human
Predicted Bovine, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 53000 Da
Antigen Region 84-111 aa
Additional Information
Gene ID 2120
Other Names Transcription factor ETV6, ETS translocation variant 6, ETS-related protein Tel1, Tel, ETV6, TEL, TEL1
Target/Specificity This ETV6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 84-111 amino acids from the N-terminal region of human ETV6.
Dilution WB~~1:2000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsETV6 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ETV6
Synonyms TEL, TEL1
Function Transcriptional repressor; binds to the DNA sequence 5'- CCGGAAGT-3'. Plays a role in hematopoiesis and malignant transformation.
Cellular Location Nucleus.
Tissue Location Ubiquitous.
Research Areas
Citations (0)
citation

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Background

ETV6 is an ETS family transcription factor. This protein contains two functional domains: a N-terminal pointed (PNT) domain that is involved in protein-protein interactions with itself and other proteins, and a C-terminal DNA-binding domain.

References

Buijs,A., et.al., Oncogene 10 (8), 1511-1519 (1995)
Golub,T.R.,et.al., Proc. Natl. Acad. Sci. U.S.A. 92 (11), 4917-4921 (1995)

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$ 365.00
$ 140.00
Cat# AP8917a
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