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B4GalT1 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - B4GalT1 Antibody (C-term) AP8892b
    All lanes : Anti-B4GalT1 Antibody (C-term) at 1:1000 dilution Lane 1: Hela, whole cell lysate Lane 2: T47D whole cell lysate ysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • FC - B4GalT1 Antibody (C-term) AP8892b
    B4GalT1 Antibody (C-term) (Cat. #AP8892b) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P15291
Other Accession P15535, P08037
Reactivity Human
Predicted Bovine, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 43920 Da
Antigen Region 329-358 aa
Additional Information
Gene ID 2683
Other Names Beta-1, 4-galactosyltransferase 1, Beta-1, 4-GalTase 1, Beta4Gal-T1, b4Gal-T1, 241-, UDP-Gal:beta-GlcNAc beta-1, 4-galactosyltransferase 1, UDP-galactose:beta-N-acetylglucosamine beta-1, 4-galactosyltransferase 1, Lactose synthase A protein, N-acetyllactosamine synthase, Nal synthase, Beta-N-acetylglucosaminylglycopeptide beta-1, 4-galactosyltransferase, Beta-N-acetylglucosaminyl-glycolipid beta-1, 4-galactosyltransferase, 241-, Processed beta-1, 4-galactosyltransferase 1, B4GALT1, GGTB2
Target/Specificity This B4GalT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-358 amino acids from the C-terminal region of human B4GalT1.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsB4GalT1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name B4GALT1 (HGNC:924)
Synonyms GGTB2
Function [Beta-1,4-galactosyltransferase 1]: The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
Cellular Location [Isoform Long]: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein Cell surface. Cell projection, filopodium {ECO:0000250|UniProtKB:P15535}. Note=Found in trans cisternae of Golgi but is mainly localized at the plasma membrane (PubMed:1714903) B4GALT1 cell surface expression is regulated by UBE2Q1 (By similarity) {ECO:0000250|UniProtKB:P15535, ECO:0000269|PubMed:1714903} [Processed beta-1,4-galactosyltransferase 1]: Secreted. Note=Soluble form found in body fluids.
Tissue Location Ubiquitously expressed, but at very low levels in fetal and adult brain
Research Areas
Citations (0)
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Background

B4GalT1 is an enzyme that participates both in glycoconjugate and lactose biosynthesis. For the first activity, the enzyme adds galactose to N-acetylglucosamine residues that are either monosaccharides or the nonreducing ends of glycoprotein carbohydrate chains. The second activity is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha-lactalbumin to catalyze UDP-galactose + D-glucose <=> UDP + lactose. The two enzymatic forms result from alternate transcription initiation sites and post-translational processing.

References

Mengle-Gaw,L., et.al., Biochem. Biophys. Res. Commun. 176 (3), 1269-1276 (1991)

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$ 365.00
$ 140.00
Cat# AP8892b
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