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CYP51A1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CYP51A1 Antibody (C-term) AP8874b
    Western blot analysis of CYP51A1 Antibody (C-term) (Cat. #AP8874b) in HL-60 cell line lysates (35ug/lane). CYP51A1 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - CYP51A1 Antibody (C-term) AP8874b
    Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with CYP51A1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q16850
Other Accession Q64654, O46420, Q8K0C4, Q4R8S6
Reactivity Human
Predicted Monkey, Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 57278 Da
Antigen Region 474-503 aa
Additional Information
Gene ID 1595
Other Names Lanosterol 14-alpha demethylase, LDM, CYPLI, Cytochrome P450 51A1, Cytochrome P450-14DM, Cytochrome P45014DM, Cytochrome P450LI, Sterol 14-alpha demethylase, CYP51A1, CYP51
Target/Specificity This CYP51A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 474-503 amino acids from the C-terminal region of human CYP51A1.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYP51A1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYP51A1 (HGNC:2649)
Synonyms CYP51
Function Sterol 14alpha-demethylase that plays a critical role in the cholesterol biosynthesis pathway, being cholesterol the major sterol component in mammalian membranes as well as a precursor for bile acid and steroid hormone synthesis (PubMed:20149798, PubMed:8619637, PubMed:9559662). Cytochrome P450 monooxygenase that catalyzes the three-step oxidative removal of the 14alpha-methyl group (C-32) of sterols such as lanosterol (lanosta-8,24-dien-3beta-ol) and 24,25- dihydrolanosterol (DHL) in the form of formate, and converts the sterols to 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol and 4,4- dimethyl-8,14-cholestadien-3beta-ol, respectively, which are intermediates of cholesterol biosynthesis (PubMed:20149798, PubMed:8619637, PubMed:9559662). Can also demethylate substrates not intrinsic to mammals, such as eburicol (24-methylene-24,25- dihydrolanosterol), but at a lower rate than DHL (PubMed:9559662).
Cellular Location Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q64654}; Single-pass membrane protein. Microsome membrane {ECO:0000250|UniProtKB:Q64654}; Single-pass membrane protein
Tissue Location Ubiquitously expressed with highest levels in testis, ovary, adrenal, prostate, liver, kidney and lung
Research Areas
Citations (0)
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Background

CYP51A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol.

References

Wang,Y., et.al., J. Biol. Chem. 283 (39), 26332-26339 (2008)

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$ 365.00
$ 140.00
Cat# AP8874b
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