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RPS8 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RPS8 Antibody (N-term) AP8831a
    Western blot analysis of RPS8 Antibody (N-term) (Cat. #AP8831a) in HL-60 cell line lysates (35ug/lane). RPS8 (arrow) was detected using the purified Pab.
    detail
  • WB - RPS8 Antibody (N-term) AP8831a
    Western blot analysis of RPS8 Antibody (N-term) (Cat. #AP8831a) in NIH-3T3 cell line lysates (35ug/lane). RPS8 (arrow) was detected using the purified Pab
    detail
  • FC - RPS8 Antibody (N-term) AP8831a
    RPS8 Antibody (N-term) (Cat. #AP8831a) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
  • CITATIONS: 2
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession P62241
Other Accession Q7SYU0, P62243, P62242, Q4R6P8, Q5E958, NP_001003, G1TJW1
Reactivity Human, Mouse
Predicted Bovine, Monkey, Rabbit, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 24205 Da
Antigen Region 1-28 aa
Additional Information
Gene ID 6202
Other Names 40S ribosomal protein S8, RPS8
Target/Specificity This RPS8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-28 amino acids from the N-terminal region of human RPS8.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRPS8 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPS8 (HGNC:10441)
Function Component of the small ribosomal subunit (PubMed:23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).
Cellular Location Cytoplasm. Membrane; Lipid-anchor. Nucleus, nucleolus. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Research Areas
Citations ( 0 )

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS8 is a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S8E family of ribosomal proteins. It is located in the cytoplasm.

References

Wool,I.G., et.al., Biochem. Cell Biol. 73 (11-12), 933-947 (1995)

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$ 182.50
$ 70.00
Cat# AP8831a
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