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CTHRC1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CTHRC1 Antibody (N-term) AP8778a
    Western blot analysis of CTHRC1 (arrow) using rabbit polyclonal CTHRC1 Antibody (N-term) (Cat. #AP8778a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CTHRC1 gene.
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  • WB - CTHRC1 Antibody (N-term) AP8778a
    All lanes : Anti-CTHRC1 Antibody (N-term) at 1:16000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - CTHRC1 Antibody (N-term) AP8778a
    Formalin-fixed and paraffin-embedded human skin tissue reacted with CTHRC1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - CTHRC1 Antibody (N-term) AP8778a
    CTHRC1 Antibody (N-term) (Cat. #AP8778a) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
  • CITATIONS: 2
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, WB, IHC-P, E
Primary Accession Q96CG8
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 26224 Da
Antigen Region 19-46 aa
Additional Information
Gene ID 115908
Other Names Collagen triple helix repeat-containing protein 1, Protein NMTC1, CTHRC1
Target/Specificity This CTHRC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-46 amino acids from the N-terminal region of human CTHRC1.
Dilution WB~~1:16000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCTHRC1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CTHRC1
Function May act as a negative regulator of collagen matrix deposition.
Cellular Location Secreted, extracellular space, extracellular matrix
Tissue Location Isoform 1 is expressed in calcified atherosclerotic plaque and chondrocyte-like cells.
Research Areas
Citations ( 0 )

Background

CTHRC1 may act as a negative regulator of collagen matrix deposition (By similarity).

References

Clark,H.F., et.al., Genome Res. 13 (10), 2265-2270 (2003)

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$ 365.00
$ 140.00
Cat# AP8778a
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