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HSPA7 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HSPA7 Antibody (Center) AP8748C
    Anti-HSPA7 Antibody (Center) at 1:2000 dilution + 293 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 70 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - HSPA7 Antibody (Center) AP8748C
    Anti-HSPA7 Antibody (Center) at 1:2000 dilution + CCRF-CEM whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 70 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - HSPA7 Antibody (Center) AP8748C
    AP8748C staining HSPA7 in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/250) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • IHC-P - HSPA7 Antibody (Center) AP8748C
    AP8748C staining HSPA7 in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/250) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • FC - HSPA7 Antibody (Center) AP8748C
    Overlay histogram showing U-2 OS cells stained with AP8748C(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP8748C, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession P48741
Other Accession P17066
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 40244 Da
Antigen Region 169-196 aa
Additional Information
Other Names Putative heat shock 70 kDa protein 7, Heat shock 70 kDa protein B, HSPA7, HSP70B
Target/Specificity This HSPA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-196 amino acids from the Central region of human HSPA7.
Dilution WB~~1:2000
IHC-P~~1:250
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSPA7 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSPA7
Synonyms HSP70B
Research Areas
Citations (0)
citation

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References

Voellmy R., et.al., Proc. Natl. Acad. Sci. U.S.A. 82:4949-4953(1985).

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$ 365.00
$ 140.00
Cat# AP8748C
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