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GSTA1 Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GSTA1 Antibody AP8697a
    All lanes : Anti-GSTA1 Antibody at 1:4000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Caco2 whole cell lysate Lane 3: LNCaP whole cell lysate Lane 4: PANC-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - GSTA1 Antibody AP8697a
    All lanes : Anti-GSTA1 Antibody at 1:4000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Caco2 whole cell lysate Lane 3: LNCaP whole cell lysate Lane 4: Human fetal lung tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P08263
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 25631 Da
Additional Information
Gene ID 2938
Other Names Glutathione S-transferase A1, GST HA subunit 1, GST class-alpha member 1, GST-epsilon, GSTA1-1, GTH1, Glutathione S-transferase A1, N-terminally processed, GSTA1
Target/Specificity This GSTA1 antibody is generated from rabbits immunized with GSTA1 recombinant protein.
Dilution WB~~1:4000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGSTA1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GSTA1
Function Glutathione S-transferase that catalyzes the nucleophilic attack of the sulfur atom of glutathione on the electrophilic groups of a wide range of exogenous and endogenous compounds (Probable). Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). It also catalyzes the isomerization of D5-androstene-3,17-dione (AD) into D4-androstene- 3,17-dione and may therefore play an important role in hormone biosynthesis (PubMed:11152686). Through its glutathione-dependent peroxidase activity toward the fatty acid hydroperoxide (13S)- hydroperoxy-(9Z,11E)-octadecadienoate/13-HPODE it is also involved in the metabolism of oxidized linoleic acid (PubMed:16624487).
Cellular Location Cytoplasm.
Tissue Location Liver.
Research Areas
Citations (0)
citation

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Background

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione.

References

Bogaards,J.J., et.al., Biochem. J. 286 (PT 2), 383-388 (1992)
Mikhal'chik,E.V., et.al., Bull. Exp. Biol. Med. 147 (6), 753-756 (2009)

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$ 182.50
$ 70.00
Cat# AP8697a
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