TSPAN1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC, IHC-P, WB, E |
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Primary Accession | O60635 |
Other Accession | NP_005718.2 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 26301 Da |
Antigen Region | 140-167 aa |
Gene ID | 10103 |
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Other Names | Tetraspanin-1, Tspan-1, Tetraspan NET-1, Tetraspanin TM4-C, TSPAN1 |
Target/Specificity | This TSPAN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-167 amino acids of human TSPAN1. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | TSPAN1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | TSPAN1 |
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Function | Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking (PubMed:30066932, PubMed:30291375). Regulates neuronal differentiation in response to NGF by facilitating NGF-mediated activation of NTRK1/TRKA receptor tyrosine kinase and subsequent downstream signaling pathways (By similarity). Plays a role in the inhibition of TNFalpha-induced apoptosis. Mechanistically, inhibits the NF-kappa-B signaling pathway by blocking phosphorylation of CHUK (PubMed:30291375). Promotes also the stability of the thiamine transporter 1/SLC19A2 in intestinal epithelial cells leading to an increase of thiamine uptake process (PubMed:21836059). |
Cellular Location | Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi- pass membrane protein |
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Provided below are standard protocols that you may find useful for product applications.
Background
TSPAN1 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility.
References
Xu,D., et.al., FASEB J. 23 (11), 3674-3681 (2009)
Berditchevski,F.et.al., J. Cell. Sci. 114 (PT 23), 4143-4151 (2001)
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