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PECI Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PECI Antibody (C-term) AP8692b
    Western blot analysis of PECI Antibody (C-term) (Cat. #AP8692b) in HepG2 cell line lysates (35ug/lane).PECI (arrow) was detected using the purified Pab.
    detail
  • WB - PECI Antibody (C-term) AP8692b
    Western blot analysis of PECI (arrow) using rabbit polyclonal PECI Antibody (C-term) (Cat. #AP8692b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PECI gene.
    detail
  • IHC-P - PECI Antibody (C-term) AP8692b
    Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PECI Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - PECI Antibody (C-term) AP8692b
    PECI Antibody (C-term) (Cat. #AP8692b) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession O75521
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 43585 Da
Antigen Region 331-358 aa
Additional Information
Gene ID 10455
Other Names Enoyl-CoA delta isomerase 2, mitochondrial, DRS-1, Delta(3), delta(2)-enoyl-CoA isomerase, D3, D2-enoyl-CoA isomerase, Diazepam-binding inhibitor-related protein 1, DBI-related protein 1, Dodecenoyl-CoA isomerase, Hepatocellular carcinoma-associated antigen 88, Peroxisomal 3, 2-trans-enoyl-CoA isomerase, pECI, Renal carcinoma antigen NY-REN-1, ECI2, DRS1, HCA88, PECI
Target/Specificity This PECI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-358 amino acids from the C-terminal region of human PECI.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPECI Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ECI2
Synonyms DRS1, HCA88, PECI {ECO:0000303|PubMed:10
Function Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates.
Cellular Location [Isoform 1]: Mitochondrion {ECO:0000250|UniProtKB:Q5XIC0}
Tissue Location Abundant in heart, skeletal muscle and liver. Expressed in CD34(+) T-cells and CD34(+) bone marrow cells
Research Areas
Citations (0)
citation

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Background

PECI is an auxiliary enzyme that catalyzes an isomerization step required for the beta-oxidation of unsaturated fatty acids.

References

Scanlan,M.J., et.al., Int. J. Cancer 83 (4), 456-464 (1999)
Suk,K., et.al., Biochim. Biophys. Acta 1454 (1), 126-131 (1999)

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$ 365.00
$ 140.00
Cat# AP8692b
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