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ATF6 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ATF6 Antibody (Center) AP8681c
    Western blot analysis of ATF6 Antibody (Center) (Cat. #AP8681c) in A2058 cell line lysates (35ug/lane). ATF6 (arrow) was detected using the purified Pab.
    detail
  • WB - ATF6 Antibody (Center) AP8681c
    Anti-ATF6 Antibody (Center) at 1:16000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 75 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - ATF6 Antibody (Center) AP8681c
    Formalin-fixed and paraffin-embedded human brain tissue reacted with ATF6 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - ATF6 Antibody (Center) AP8681c
    ATF6 Antibody (Center) (Cat. #AP8681c) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession P18850
Other Accession G3V909, F6VAN0
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 74585 Da
Antigen Region 350-377 aa
Additional Information
Gene ID 22926
Other Names Cyclic AMP-dependent transcription factor ATF-6 alpha, cAMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, ATF6-alpha, Processed cyclic AMP-dependent transcription factor ATF-6 alpha, ATF6
Target/Specificity This ATF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 350-377 amino acids of human ATF6.
Dilution WB~~1:16000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATF6 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATF6
Function [Cyclic AMP-dependent transcription factor ATF-6 alpha]: Precursor of the transcription factor form (Processed cyclic AMP- dependent transcription factor ATF-6 alpha), which is embedded in the endoplasmic reticulum membrane (PubMed:10564271, PubMed:11158310, PubMed:11779464). Endoplasmic reticulum stress promotes processing of this form, releasing the transcription factor form that translocates into the nucleus, where it activates transcription of genes involved in the unfolded protein response (UPR) (PubMed:10564271, PubMed:11158310, PubMed:11779464).
Cellular Location Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus membrane; Single-pass type II membrane protein. Note=Translocates from the endoplasmic reticulum to the Golgi, where it is processed.
Tissue Location Ubiquitous..
Research Areas
Citations ( 0 )

Background

ATF6 is an endoplasmic reticulum (ER) stress-regulated transmembrane transcription factor that activates the transcription of ER molecules.

References

Zhu,C., et.al., Mol. Cell. Biol. 17 (9), 4957-4966 (1997)
Haze,K., et.al., Mol. Biol. Cell 10 (11), 3787-3799 (1999)

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$ 365.00
$ 140.00
Cat# AP8681c
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