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GAPDHS Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 2
- PROTOCOLS
- BACKGROUND
Application 
| FC, IHC-P, WB, E |
|---|---|
| Primary Accession | O14556 |
| Other Accession | Q4R3T1 |
| Reactivity | Human |
| Predicted | Monkey |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 44501 Da |
| Antigen Region | 104-134 aa |
| Gene ID | 26330 |
|---|---|
| Other Names | Glyceraldehyde-3-phosphate dehydrogenase, testis-specific, Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2, GAPDH-2, Spermatogenic glyceraldehyde-3-phosphate dehydrogenase, GAPDHS, GAPD2, GAPDH2, GAPDS |
| Target/Specificity | This GAPDHS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-134 amino acids from the Central region of human GAPDHS. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | GAPDHS Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GAPDHS |
|---|---|
| Synonyms | GAPD2, GAPDH2, GAPDS |
| Function | May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility (By similarity). |
| Cellular Location | Cytoplasm. |
| Tissue Location | Testis specific. |
Provided below are standard protocols that you may find useful for product applications.
Background
GAPDHS is a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility.
References
Welch,J.E., et.al., J. Androl. 21 (2), 328-338 (2000) Goodwin,L.O., et.al., Mol. Hum. Reprod. 6 (2), 127-136 (2000) Benham,F.J. et.al., Genomics 5 (2), 209-214 (1989)
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