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WNT5B Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - WNT5B Antibody (Center) AP8603C
    Western blot analysis of WNT5B Antibody (Center) (Cat. #AP8603c) in mouse bladder tissue lysates (35ug/lane). WNT5B (arrow) was detected using the purified Pab.
    detail
  • IHC-P - WNT5B Antibody (Center) AP8603C
    Formalin-fixed and paraffin-embedded human skeletal muscle with WNT5B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - WNT5B Antibody (Center) AP8603C
    Flow cytometric analysis of widr cells using WNT5B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q9H1J7
Other Accession P33945, P22726, P31286, Q9QXQ7, Q27Q52, P22725, P41221
Reactivity Human, Mouse
Predicted Rabbit, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 40323 Da
Antigen Region 153-182 aa
Additional Information
Gene ID 81029
Other Names Protein Wnt-5b, WNT5B
Target/Specificity This WNT5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-182 amino acids from the Central region of human WNT5B.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWNT5B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WNT5B
Function Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity).
Cellular Location Secreted, extracellular space, extracellular matrix
Research Areas
Citations (0)
citation

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Background

WNT5B is a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively.

References

Saitoh,T. et.al., Int. J. Mol. Med. 10 (3), 345-349 (2002) Yates,K.E. et.al., Osteoarthr. Cartil. 12 (6), 497-505 (2004)

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$ 365.00
$ 140.00
Cat# AP8603C
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