PPM1D Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 8
- PROTOCOLS
- BACKGROUND
Application ![]()
| WB, E |
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Primary Accession | O15297 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 66675 Da |
Antigen Region | 571-602 aa |
Gene ID | 8493 |
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Other Names | Protein phosphatase 1D, Protein phosphatase 2C isoform delta, PP2C-delta, Protein phosphatase magnesium-dependent 1 delta, p53-induced protein phosphatase 1, PPM1D, WIP1 |
Target/Specificity | This PPM1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 571-602 amino acids from the C-terminal region of human PPM1D. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | PPM1D Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PPM1D |
---|---|
Synonyms | WIP1 |
Function | Involved in the negative regulation of p53 expression (PubMed:23242139). Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins (PubMed:15870257, PubMed:16311512). Mediates MAPK14 dephosphorylation and inactivation (PubMed:21283629). Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity (By similarity). |
Cellular Location | Nucleus. Cytoplasm, cytosol |
Tissue Location | Expressed in fetal and adult brain. Also detected in fetal liver and skeletal muscle, but not in their adult counterparts. |

Provided below are standard protocols that you may find useful for product applications.
Background
PPM1D is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. Expression of this PPM1D gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. The PPM1D gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development.

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