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YWHAZ Antibody (D231)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P63104 |
| Other Accession | P63102, P63101, Q5ZKC9, P63103, P29361 |
| Reactivity | Human, Mouse |
| Predicted | Bovine, Chicken, Rat, Sheep |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 27745 Da |
| Antigen Region | 216-245 aa |
| Gene ID | 7534 |
|---|---|
| Other Names | 14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ |
| Target/Specificity | This YWHAZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-245 amino acids from human YWHAZ. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | YWHAZ Antibody (D231) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | YWHAZ |
|---|---|
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:14578935, PubMed:15071501, PubMed:15644438, PubMed:16376338, PubMed:16959763, PubMed:31024343, PubMed:9360956). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed:35662396). Binding generally results in the modulation of the activity of the binding partner (PubMed:35662396). Promotes cytosolic retention and inactivation of TFEB transcription factor by binding to phosphorylated TFEB (PubMed:35662396). Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation (PubMed:16959763). In neurons, regulates spine maturation through the modulation of ARHGEF7 activity (By similarity). |
| Cellular Location | Cytoplasm. Melanosome. Note=Located to stage I to stage IV melanosomes. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
YWHAZ belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for the gene. Both variants encode the same protein, however, they are differentially expressed in hematopoietic cells.
References
Powell, D.W., et al., Mol. Cell. Biol. 23(15):5376-5387 (2003).
Zhu, P., et al., Biochem. Biophys. Res. Commun. 301(4):991-999 (2003).
Li, Y., et al., J. Biol. Chem. 277(47):44593-44596 (2002).
Wang, H., et al., J. Clin. Endocrinol. Metab. 87(6):2629-2634 (2002).
Nellist, M., et al., J. Biol. Chem. 277(42):39417-39424 (2002).
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