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YWHAZ Antibody (D231)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - YWHAZ Antibody (D231) AP8152b
    Western blot analysis of hYWHAZ-D231 (Cat. #AP8152b) in mouse brain tissue and MCF-7 cell line lysates (35ug/lane). YWHAZ (arrow) was detected using the purified Pab.
    detail
  • IHC-P - YWHAZ Antibody (D231) AP8152b
    Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with 14-3-3 protein zeta/delta antibody (C-term) (Cat. #AP8152b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P63104
Other Accession P63102, P63101, Q5ZKC9, P63103, P29361
Reactivity Human, Mouse
Predicted Bovine, Chicken, Rat, Sheep
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 27745 Da
Antigen Region 216-245 aa
Additional Information
Gene ID 7534
Other Names 14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ
Target/Specificity This YWHAZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-245 amino acids from human YWHAZ.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsYWHAZ Antibody (D231) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name YWHAZ
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:14578935, PubMed:15071501, PubMed:15644438, PubMed:16376338, PubMed:16959763, PubMed:31024343, PubMed:9360956). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed:35662396). Binding generally results in the modulation of the activity of the binding partner (PubMed:35662396). Promotes cytosolic retention and inactivation of TFEB transcription factor by binding to phosphorylated TFEB (PubMed:35662396). Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation (PubMed:16959763). In neurons, regulates spine maturation through the modulation of ARHGEF7 activity (By similarity).
Cellular Location Cytoplasm. Melanosome. Note=Located to stage I to stage IV melanosomes.
Research Areas
Citations (0)
citation

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Background

YWHAZ belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for the gene. Both variants encode the same protein, however, they are differentially expressed in hematopoietic cells.

References

Powell, D.W., et al., Mol. Cell. Biol. 23(15):5376-5387 (2003).
Zhu, P., et al., Biochem. Biophys. Res. Commun. 301(4):991-999 (2003).
Li, Y., et al., J. Biol. Chem. 277(47):44593-44596 (2002).
Wang, H., et al., J. Clin. Endocrinol. Metab. 87(6):2629-2634 (2002).
Nellist, M., et al., J. Biol. Chem. 277(42):39417-39424 (2002).

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$ 182.50
$ 70.00
Cat# AP8152b
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