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PFKP Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 3
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q01813 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 85596 Da |
| Antigen Region | 754-784 aa |
| Gene ID | 5214 |
|---|---|
| Other Names | ATP-dependent 6-phosphofructokinase, platelet type {ECO:0000255|HAMAP-Rule:MF_03184}, ATP-PFK {ECO:0000255|HAMAP-Rule:MF_03184}, PFK-P, 27111 {ECO:0000255|HAMAP-Rule:MF_03184}, 6-phosphofructokinase type C, Phosphofructo-1-kinase isozyme C, PFK-C, Phosphohexokinase {ECO:0000255|HAMAP-Rule:MF_03184}, PFKP, PFKF |
| Target/Specificity | This PFKP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 754-784 amino acids from the C-terminal region of human PFKP. |
| Dilution | WB~~1:8000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PFKP Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PFKP |
|---|---|
| Synonyms | PFKF |
| Function | Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. |
| Cellular Location | Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03184}. |
Provided below are standard protocols that you may find useful for product applications.
Background
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).
References
Adam, G.C., et al., Mol. Cell Proteomics 1(10):828-835 (2002).
Bonaldo, M.F., et al., Genome Res. 6(9):791-806 (1996).
Morrison, N., et al., Hum. Genet. 89(1):105-106 (1992).
Simpson, C.J., et al., Biochem. Biophys. Res. Commun. 180(1):197-203 (1991).
Nakajima, H., et al., FEBS Lett. 223(1):113-116 (1987).
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