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ULK2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ULK2 Antibody (C-term) AP8105b
    Western blot analysis of ULK2 Antibody (C-term) (Cat.# AP8105b) in mouse brain tissue lysates (35ug/lane). ULK2(arrow) was detected using the purified Pab.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8IYT8
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 112694 Da
Antigen Region 612-643 aa
Additional Information
Gene ID 9706
Other Names Serine/threonine-protein kinase ULK2, Unc-51-like kinase 2, ULK2, KIAA0623
Target/Specificity This ULK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 612-643 amino acids from the C-terminal region of human ULK2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsULK2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ULK2
Synonyms KIAA0623
Function Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and a negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation: may govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons.
Cellular Location Cytoplasmic vesicle membrane; Peripheral membrane protein. Note=Localizes to pre-autophagosomal membrane
Research Areas
Citations (0)
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Background

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Two human homologs of the yeast autophagy-specific kinase exist: ULK1(APG1) and ULK2. APG1 plays a critical role in regulating key elements of the autophagy pathway. APG1 stimulates autophagy, leading to autophagy-dependent restriction of cell growth and ultimately cell apoptosis at high levels of activity, and is a negative regulator of mTOR signaling.

References

Yan, J., et al., Oncogene 18(43):5850-5859 (1999).

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$ 365.00
$ 140.00
Cat# AP8105b
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