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PAK2 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q13177 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 58043 Da |
| Antigen Region | 192-222 aa |
| Gene ID | 5062 |
|---|---|
| Other Names | Serine/threonine-protein kinase PAK 2, Gamma-PAK, PAK65, S6/H4 kinase, p21-activated kinase 2, PAK-2, p58, PAK-2p27, p27, PAK-2p34, p34, C-t-PAK2, PAK2 |
| Target/Specificity | This PAK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-222 amino acids from the N-terminal region of human PAK2. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PAK2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PAK2 |
|---|---|
| Function | Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation (PubMed:12853446, PubMed:16617111, PubMed:19273597, PubMed:19923322, PubMed:33693784, PubMed:7744004, PubMed:9171063). Acts as a downstream effector of the small GTPases CDC42 and RAC1 (PubMed:7744004). Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues (PubMed:7744004). Full- length PAK2 stimulates cell survival and cell growth (PubMed:7744004). Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration (PubMed:21317288). Phosphorylates JUN and plays an important role in EGF-induced cell proliferation (PubMed:21177766). Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP (PubMed:21724829). Phosphorylates CASP7, thereby preventing its activity (PubMed:21555521, PubMed:27889207). Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis (PubMed:19273597, PubMed:19923322). On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway (PubMed:12853446, PubMed:16617111, PubMed:9171063). Caspase-activated PAK2 phosphorylates MKNK1 and reduces cellular translation (PubMed:15234964). |
| Cellular Location | [Serine/threonine-protein kinase PAK 2]: Cytoplasm Nucleus Note=MYO18A mediates the cellular distribution of the PAK2-ARHGEF7-GIT1 complex to the inner surface of the cell membrane |
| Tissue Location | Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen |
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Provided below are standard protocols that you may find useful for product applications.
Background
PAK2, a member of the STE20 subfamily of Ser/Thr protein kinases, acts on a variety of targets. It phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. PAK2 interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Expression is ubiquitous, with higher levels seen in skeletal muscle, ovary, thymus and spleen. PAK2 is autophosphorylated when activated by CDC42/p21. The protein structure contains 1 CRIB domain.
References
Benner, G.E., et al., J. Biol. Chem. 270(36):21121-21128 (1995).
Martin, G.A., et al., EMBO J. 14(9):1970-1978 (1995).
Martin, G.A., et al., EMBO J. 14 (17), 4385 (1995).
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