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CDC25C Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CDC25C Antibody (Center) AP7862d
    Western blot analysis of CDC25C Antibody (Center) (Cat. #AP7862d) in NIH-3T3 cell line lysates (35ug/lane). CDC25C (arrow) was detected using the purified Pab.
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P30307
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 53365 Da
Antigen Region 114-142 aa
Additional Information
Gene ID 995
Other Names M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25C, CDC25C
Target/Specificity This CDC25C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 114-142 amino acids from the Central region of human CDC25C.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCDC25C Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDC25C
Function Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle (PubMed:8119945). When phosphorylated, highly effective in activating G2 cells into prophase (PubMed:8119945). Directly dephosphorylates CDK1 and activates its kinase activity (PubMed:8119945).
Cellular Location Nucleus
Research Areas
Citations (0)
citation

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Background

CDC25C is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest.

References

Varmeh,S., Mol. Cancer Ther. 7 (12), 3789-3799 (2008)
Long,J.M., Cell Cycle 7 (19), 3062-3073 (2008)
Bonnet,J., Cell Cycle 7 (13), 1991-1998 (2008)

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$ 182.50
$ 70.00
Cat# AP7862d
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