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ACHE Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - ACHE Antibody (N-term) AP7853a
    Confocal immunofluorescent analysis of ACHE Antibody (N-term)(Cat#AP7853a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - ACHE Antibody (N-term) AP7853a
    ACHE Antibody (N-term) (Cat. #AP7853a) western blot analysis in Jurkat,Raji,Y79 cell line lysates (35ug/lane).This demonstrates the ACHE antibody detected the ACHE protein (arrow).
    detail
  • IHC-P - ACHE Antibody (N-term) AP7853a
    Formalin-fixed and paraffin-embedded human brain tissue reacted with ACHE antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - ACHE Antibody (N-term) AP7853a
    ACHE Antibody (N-term) (Cat. #AP7853a) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
Confocal immunofluorescent analysis of ACHE Antibody (N-term)(Cat#AP7853a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, FC, IHC-P, E
Primary Accession P22303
Reactivity Human
Predicted Guinea Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 67796 Da
Antigen Region 147-175 aa
Additional Information
Gene ID 43
Other Names Acetylcholinesterase, AChE, ACHE
Target/Specificity This ACHE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-175 amino acids from the N-terminal region of human ACHE.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACHE Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACHE (HGNC:108)
Function Hydrolyzes rapidly the acetylcholine neurotransmitter released into the synaptic cleft allowing to terminate the signal transduction at the neuromuscular junction. Role in neuronal apoptosis.
Cellular Location Synapse. Secreted. Cell membrane; Peripheral membrane protein [Isoform H]: Cell membrane; Lipid- anchor, GPI-anchor; Extracellular side
Tissue Location Isoform H is highly expressed in erythrocytes.
Research Areas
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$ 385.00
Cat# AP7853a
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