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BRAF Antibody (T400)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P15056 |
| Other Accession | P28028 |
| Reactivity | Human, Mouse |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 84437 Da |
| Antigen Region | 379-408 aa |
| Gene ID | 673 |
|---|---|
| Other Names | Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF, BRAF1, RAFB1 |
| Target/Specificity | This BRAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 379-408 amino acids from human BRAF. |
| Dilution | WB~~1:500 IHC-P~~1:100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | BRAF Antibody (T400) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | BRAF (HGNC:1097) |
|---|---|
| Synonyms | BRAF1, RAFB1 |
| Function | Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). Phosphorylates PFKFB2 (PubMed:36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179). |
| Cellular Location | Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes. |
| Tissue Location | Brain and testis. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.
References
Hingorani, S.R., et al., Cancer Res. 63(17):5198-5202 (2003).
Lee, J.W., et al., Br. J. Cancer 89(10):1958-1960 (2003).
Davies, H., et al., Nature 417(6892):949-954 (2002).
Naoki, K., et al., Cancer Res. 62(23):7001-7003 (2002).
Stephens, R.M., et al., Mol. Cell. Biol. 12(9):3733-3742 (1992).
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