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BRAF Antibody (T400)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - BRAF Antibody (T400) AP7810E
    All lanes : Anti-BRAF Antibody (T400) at 1:500-2000 dilution Lane 1: K562 whole cell lysate Lane 2: Raji whole cell lysate Lane 3: T47D whole cell lysate Lane 4: A20 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - BRAF Antibody (T400) AP7810E
    Anti-BRAF Antibody (T400) at 1:500 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - BRAF Antibody (T400) AP7810E
    Immunohistochemical analysis of AP7810E on paraffin-embedded Human brain tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • IHC-P - BRAF Antibody (T400) AP7810E
    Immunohistochemical analysis of AP7810E on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P15056
Other Accession P28028
Reactivity Human, Mouse
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 84437 Da
Antigen Region 379-408 aa
Additional Information
Gene ID 673
Other Names Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF, BRAF1, RAFB1
Target/Specificity This BRAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 379-408 amino acids from human BRAF.
Dilution WB~~1:500
IHC-P~~1:100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBRAF Antibody (T400) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BRAF (HGNC:1097)
Synonyms BRAF1, RAFB1
Function Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). Phosphorylates PFKFB2 (PubMed:36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179).
Cellular Location Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
Tissue Location Brain and testis.
Research Areas
Citations (0)
citation

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Background

BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.

References

Hingorani, S.R., et al., Cancer Res. 63(17):5198-5202 (2003).
Lee, J.W., et al., Br. J. Cancer 89(10):1958-1960 (2003).
Davies, H., et al., Nature 417(6892):949-954 (2002).
Naoki, K., et al., Cancer Res. 62(23):7001-7003 (2002).
Stephens, R.M., et al., Mol. Cell. Biol. 12(9):3733-3742 (1992).

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$ 182.50
$ 70.00
Cat# AP7810E
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