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NRAS Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, FC, E |
|---|---|
| Primary Accession | P01111 |
| Other Accession | Q04970, Q2MJK3, P08556, Q5F352 |
| Reactivity | Human, Mouse, Rat |
| Predicted | Chicken, Pig |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 21229 Da |
| Antigen Region | 72-101 aa |
| Gene ID | 4893 |
|---|---|
| Other Names | GTPase NRas, Transforming protein N-Ras, NRAS, HRAS1 |
| Target/Specificity | This NRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-101 amino acids from the N-terminal region of human NRAS. |
| Dilution | IF~~1:10~50 WB~~1:1000 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NRAS Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NRAS |
|---|---|
| Synonyms | HRAS1 |
| Function | Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. |
| Cellular Location | Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor Note=Shuttles between the plasma membrane and the Golgi apparatus |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
NRAS is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. This protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in the gene encoding this protein are a cause of juvenile myelomonocytic leukemia (JMML).
References
Smalley,K.S., Cancer Res. 68 (14), 5743-5752 (2008)
Banerji,U., Mol. Cancer Ther. 7 (4), 737-739 (2008)
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