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APOA4 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - APOA4 Antibody (N-term) AP7591a
    Western blot analysis of APOA4 Antibody (N-term) (Cat.#AP7591a) in Jurkat, K562, NCI-H460 cell line lysates (35ug/lane). APOA4 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - APOA4 Antibody (N-term) AP7591a
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with APOA4 antibody (N-term) (Cat.#AP7591a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P06727
Other Accession P33621, A8MSL6
Reactivity Human
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 45372 Da
Antigen Region 49-79 aa
Additional Information
Gene ID 337
Other Names Apolipoprotein A-IV, Apo-AIV, ApoA-IV, Apolipoprotein A4, APOA4
Target/Specificity This APOA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-79 amino acids from the N-terminal region of human APOA4.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAPOA4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name APOA4
Function May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.
Cellular Location Secreted.
Tissue Location Synthesized primarily in the intestine and secreted in plasma
Research Areas
Citations (0)
citation

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Background

The primary translation product of the APOA4 gene is a 396-residue preprotein which after proteolytic processing is secreted into its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.

References

Chien,K.L., Clin. Chim. Acta 388 (1-2), 78-83 (2008)
Bai,H., Acta Cardiol 63 (3), 315-322 (2008)

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$ 365.00
$ 140.00
Cat# AP7591a
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