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SOD2 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SOD2 Antibody AP7579a
    Western blot analysis of anti-SOD2 Pab (Cat.#AP7979a) in K562 cell line lysates (35ug/lane). SOD2(arrow) was detected using the purified Pab.
    detail
  • FC - SOD2 Antibody AP7579a
    SOD2 Antibody (Cat. #AP7579a) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
  • CITATIONS: 2
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession P04179
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 24750 Da
Additional Information
Gene ID 6648
Other Names Superoxide dismutase [Mn], mitochondrial, SOD2
Target/Specificity This SOD2 antibody is generated from rabbits immunized with human recombinant SD protein.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSOD2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SOD2
Function Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Cellular Location Mitochondrion matrix.
Research Areas
Citations ( 0 )

Background

SOD2 is a member of the iron/manganese superoxide dismutase family. It is a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer.

References

Fabre,E.E., Am. J. Clin. Nutr. 87 (5), 1504-1512 (2008) Kaewpila,S., Cancer Res. 68 (8), 2781-2788 (2008) Flekac,M., (er) BMC Med. Genet. 9, 30 (2008)

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$ 182.50
$ 70.00
Cat# AP7579a
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