ACAT1 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
---|---|
Primary Accession | P24752 |
Other Accession | Q8HXY6 |
Reactivity | Human, Mouse |
Predicted | Monkey |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 45200 Da |
Antigen Region | 296-329 aa |
Gene ID | 38 |
---|---|
Other Names | Acetyl-CoA acetyltransferase, mitochondrial, Acetoacetyl-CoA thiolase, T2, ACAT1, ACAT, MAT |
Target/Specificity | This ACAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-329 amino acids from the C-terminal region of human ACAT1. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | ACAT1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ACAT1 |
---|---|
Synonyms | ACAT, MAT |
Function | This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA (PubMed:1715688, PubMed:7728148, PubMed:9744475). Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms (PubMed:1715688, PubMed:7728148, PubMed:9744475). The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA (PubMed:17371050). Thereby, it plays a major role in ketone body metabolism (PubMed:1715688, PubMed:17371050, PubMed:7728148, PubMed:9744475). |
Cellular Location | Mitochondrion. |
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Provided below are standard protocols that you may find useful for product applications.
Background
ACAT1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.
References
Locke,J.A.,Prostate 68 (1), 20-33 (2008)
Guo,Z.Y.,Biochemistry 46 (35), 10063-10071 (2007)
Haapalainen,A.M.,Biochemistry 46 (14), 4305-4321 (2007)
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