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> home > Products > Primary Antibodies > Antibody Collections > Anti-Wnt-Pathway (plus Lgr5) Antibodies > NLK-T286 Antibody (Center)
NLK-T286 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IF, FC, WB, E |
|---|---|
| Primary Accession | Q9UBE8 |
| Other Accession | D3ZSZ3, O54949, E1BMN8, NP_057315.3 |
| Reactivity | Human |
| Predicted | Bovine, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 58283 Da |
| Antigen Region | 275-300 aa |
| Gene ID | 51701 |
|---|---|
| Other Names | Serine/threonine-protein kinase NLK, Nemo-like kinase, Protein LAK1, NLK, LAK1 {ECO:0000312|EMBL:AAD560131} |
| Target/Specificity | This NLK-T286 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-300 amino acids from the Central region of human NLK-T286. |
| Dilution | IF~~1:10~50 WB~~1:1000 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NLK-T286 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NLK |
|---|---|
| Synonyms | LAK1 {ECO:0000312|EMBL:AAD56013.1} |
| Function | Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination (PubMed:12482967, PubMed:14960582, PubMed:15004007, PubMed:15764709, PubMed:20061393, PubMed:20874444, PubMed:21454679). Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2 (PubMed:15004007, PubMed:15764709). Negative regulator of the canonical Wnt/beta-catenin signaling pathway (PubMed:12482967). Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1 (PubMed:21454679). Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes (PubMed:12482967, PubMed:21454679). Negative regulator of the Notch signaling pathway (PubMed:20118921). Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1 (PubMed:20118921). Negative regulator of the MYB family of transcription factors (PubMed:15082531). Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP (PubMed:15082531). Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself (PubMed:15082531). Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1 (PubMed:15004007, PubMed:15764709). Upon IL1B stimulus, cooperates with ATF5 to activate the transactivation activity of C/EBP subfamily members (PubMed:25512613). Phosphorylates ATF5 but also stabilizes ATF5 protein levels in a kinase-independent manner (PubMed:25512613). Acts as an inhibitor of the mTORC1 complex in response to osmotic stress by mediating phosphorylation of RPTOR, thereby preventing recruitment of the mTORC1 complex to lysosomes (PubMed:26588989). |
| Cellular Location | Nucleus {ECO:0000250|UniProtKB:O54949}. Cytoplasm {ECO:0000250|UniProtKB:O54949}. Note=Predominantly nuclear. A smaller fraction is cytoplasmic. {ECO:0000250|UniProtKB:O54949} |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
Kim, S., et al. J. Biol. Chem. 285(11):8122-8129(2010)
Ishitani, T., et al. Nat. Cell Biol. 12(3):278-285(2010)
Shi, Y., et al. Mol. Cell. Biochem. 333 (1-2), 293-298 (2010) :
Emami, K.H., et al. Prostate 69(14):1481-1492(2009)
Saijo, K., et al. Cell 137(1):47-59(2009)
Wistow, G., et al. Mol. Vis. 8, 196-204 (2002) :
Kortenjann, M., et al. Gene 278 (1-2), 161-165 (2001) :
Kehrer-Sawatzki, H., et al. Gene 251(1):63-71(2000)
Brott, B.K., et al. Proc. Natl. Acad. Sci. U.S.A. 95(3):963-968(1998)
Volorio, S., et al. DNA Seq. 9 (5-6), 307-315 (1998) :
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$ 365.00
$ 140.00
Cat# AP7545c
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