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GPT Antibody (N-term R133)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P24298 |
| Other Accession | A4IFH5 |
| Reactivity | Human, Mouse, Rat |
| Predicted | Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 54637 Da |
| Antigen Region | 118-144 aa |
| Gene ID | 2875 |
|---|---|
| Other Names | Alanine aminotransferase 1, ALT1, Glutamate pyruvate transaminase 1, GPT 1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1, GPT, AAT1, GPT1 |
| Target/Specificity | This GPT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-144 amino acids from the N-terminal region of human GPT. |
| Dilution | WB~~1:1000 IHC-P~~1:100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | GPT Antibody (N-term R133) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GPT |
|---|---|
| Synonyms | AAT1, GPT1 |
| Function | Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity). |
| Cellular Location | Cytoplasm. |
| Tissue Location | Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue |
Provided below are standard protocols that you may find useful for product applications.
Background
GPT(Glutamate-pyruvate transaminase), also known as alanine aminotransferase, catalyzes the reversible conversion of L-alanine and alpha-ketoglutarate to L-glutamate and pyruvate. This protein has 2 distinct molecular and genetic forms: one cytoplasmic (soluble) (GPT1) and one mitochondrial (GPT2). See ALTQTL1 and ALTQTL2 for information on quantitative trait loci influencing the plasma level of alanine aminotransferase.
References
Yang R.-Z., Blaileanu G.Genomics 79:445-450(2002)
Ishiguro M., Takio K.Biochemistry 30:10451-10457(1991)
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