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IDH1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - IDH1 Antibody (N-term) AP7454A
    Confocal immunofluorescent analysis of IDH1 Antibody (N-term)(Cat#AP7454a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - IDH1 Antibody (N-term) AP7454A
    Western blot analysis of IDH1 Antibody (N-term) (Cat.#AP7454a) in mouse liver tissue and 293 cell line lysates (35ug/lane). IDH1 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - IDH1 Antibody (N-term) AP7454A
    Formalin-fixed and paraffin-embedded human brain reacted with IDH1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - IDH1 Antibody (N-term) AP7454A
    Flow cytometric analysis of 293 cells using IDH1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, IF, E
Primary Accession O75874
Other Accession P41562, O88844, Q9XSG3, Q6XUZ5
Reactivity Human, Mouse
Predicted Bovine, Rat, Sheep
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 46659 Da
Antigen Region 63-90 aa
Additional Information
Gene ID 3417
Other Names Isocitrate dehydrogenase [NADP] cytoplasmic, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, IDH1, PICD
Target/Specificity This IDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-90 amino acids from the N-terminal region of human IDH1.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIDH1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IDH1
Synonyms PICD
Function Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed:10521434, PubMed:19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed:10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).
Cellular Location Cytoplasm, cytosol. Peroxisome
Research Areas
Citations (0)
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Background

IDH1 belongs to two distinct subclasses. The protein is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.

References

Geisbrecht B.V., Gould S.J.J. Biol. Chem. 274:30527-30533(1999)
Xu X., Zhao J., Xu Z.J. Biol. Chem. 279:33946-33957(2004)
Bleeker F.E., Lamba S.Hum. Mutat. 30:7-11(2009)

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$ 365.00
$ 140.00
Cat# AP7454A
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