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ADAM9 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ADAM9 Antibody (C-term) AP7437b
    Western blot analysis of ADAM9 Antibody (C-term) (Cat. #AP7437b) in MDA-MB231 cell line lysates (35ug/lane). ADAM9 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - ADAM9 Antibody (C-term) AP7437b
    Formalin-fixed and paraffin-embedded human lung carcinoma reacted with ADAM9 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - ADAM9 Antibody (C-term) AP7437b
    ADAM9 Antibody (C-term) (Cat. #AP7437b) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
  • CITATIONS: 1
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q13443
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 90556 Da
Antigen Region 704-733 aa
Additional Information
Gene ID 8754
Other Names Disintegrin and metalloproteinase domain-containing protein 9, ADAM 9, 3424-, Cellular disintegrin-related protein, Meltrin-gamma, Metalloprotease/disintegrin/cysteine-rich protein 9, Myeloma cell metalloproteinase, ADAM9, KIAA0021, MCMP, MDC9, MLTNG
Target/Specificity This ADAM9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 704-733 amino acids from the C-terminal region of human ADAM9.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADAM9 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADAM9
Synonyms KIAA0021, MCMP, MDC9, MLTNG
Function Metalloprotease that cleaves and releases a number of molecules with important roles in tumorigenesis and angiogenesis, such as TEK, KDR, EPHB4, CD40, VCAM1 and CDH5. May mediate cell-cell, cell- matrix interactions and regulate the motility of cells via interactions with integrins.
Cellular Location [Isoform 1]: Cell membrane; Single-pass type I membrane protein
Tissue Location Widely expressed. Expressed in chondrocytes. Isoform 2 is highly expressed in liver and heart
Research Areas
Citations ( 0 )

Background

ADAM9 is a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This protein interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor.

References

Weskamp G., Kraetzschmar J., Reid M.S.J. Cell Biol. 132:717-726(1996)
Hotoda N., Koike H.Biochem. Biophys. Res. Commun. 293:800-805(2002)
McKie N., Edwards T., Dallas D.J.Biochem. Biophys. Res. Commun. 230:335-339(1997)

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$ 365.00
$ 140.00
Cat# AP7437b
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