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ANXA2 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ANXA2 Antibody (N-term) AP7420a
    Western blot analysis of ANXA2 (arrow) using rabbit polyclonal ANXA2 Antibody (N-term) (Cat.#AP7420a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ANXA2 gene.
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  • WB - ANXA2 Antibody (N-term) AP7420a
    Western blot analysis of anti-ANXA2 Antibody (N-term) (Cat.#AP7420a) in mouse lung tissue lysates (35ug/lane). ANXA2(arrow) was detected using the purified Pab.
    detail
  • WB - ANXA2 Antibody (N-term) AP7420a
    ANXA2 Antibody (N-term) (Cat. #AP7420a) western blot analysis in 293,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the ANXA2 antibody detected the ANXA2 protein (arrow).
    detail
  • WB - ANXA2 Antibody (N-term) AP7420a
    All lanes : Anti-ANXA2 Antibody (N-term) at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: NIH/3T3 whole cell lysates Lane 4: SH-SY5Y whole cell lysates Lane 5: SK-BR-3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • IHC-P - ANXA2 Antibody (N-term) AP7420a
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ANXA2 antibody (N-term) (Cat.#AP7420a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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  • IHC-P - ANXA2 Antibody (N-term) AP7420a
    AP7420a staining ANXA2 in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P07355
Other Accession A6NMY6, Q07936, P19620, P04272, A2SW69
Reactivity Human, Mouse
Predicted Bovine, Pig, Rat, Sheep
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 38604 Da
Antigen Region 19-49 aa
Additional Information
Gene ID 302
Other Names Annexin A2, Annexin II, Annexin-2, Calpactin I heavy chain, Calpactin-1 heavy chain, Chromobindin-8, Lipocortin II, Placental anticoagulant protein IV, PAP-IV, Protein I, p36, ANXA2, ANX2, ANX2L4, CAL1H, LPC2D
Target/Specificity This ANXA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-49 amino acids from the N-terminal region of human ANXA2.
Dilution WB~~1:2000
IHC-P~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsANXA2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ANXA2
Synonyms ANX2, ANX2L4, CAL1H, LPC2D
Function Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9 (PubMed:18799458, PubMed:24808179, PubMed:22848640).
Cellular Location Secreted, extracellular space, extracellular matrix, basement membrane. Melanosome. Note=In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism
Research Areas
Citations ( 0 )

Background

ANXA2 is a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption.

References

He,K.L., J. Biol. Chem. 283 (28), 19192-19200 (2008) Tamma,G., Pflugers Arch. 456 (4), 729-736 (2008) Gou,D., J. Biol. Chem. 283 (19), 13156-13164 (2008)

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$ 182.50
$ 70.00
Cat# AP7420a
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