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TECPR1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - TECPR1 Antibody (C-term) AP7391b
    All lanes : Anti-TECPR1 Antibody (C-term) at 1:500 dilution Lane 1: HUVEC whole cell lysate Lane 2: A375 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 130 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - TECPR1 Antibody (C-term) AP7391b
    Immunohistochemical analysis of AP7391b on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • FC - TECPR1 Antibody (C-term) AP7391b
    TECPR1 Antibody (C-term) (Cat. #AP7391b) flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, FC, WB, E
Primary Accession Q7Z6L1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 129696 Da
Antigen Region 1094-1122 aa
Additional Information
Gene ID 25851
Other Names Tectonin beta-propeller repeat-containing protein 1, TECPR1, KIAA1358
Target/Specificity This TECPR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1094-1122 amino acids from the C-terminal region of human TECPR1.
Dilution WB~~1:500
IHC-P~~1:100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTECPR1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TECPR1
Synonyms KIAA1358
Function Tethering factor involved in autophagy. Involved in autophagosome maturation by promoting the autophagosome fusion with lysosomes: acts by associating with both the ATG5-ATG12 conjugate and phosphatidylinositol-3-phosphate (PtdIns(3)P) present at the surface of autophagosomes. Also involved in selective autophagy against bacterial pathogens, by being required for phagophore/preautophagosomal structure biogenesis and maturation.
Cellular Location Cytoplasmic vesicle, autophagosome membrane. Lysosome membrane. Note=Localizes to Lysosome membranes, and binds PtdIns(3)P at the surface of autophagosome. Localizes to autolysosomes, a vesicle formed by the fusion between autophagosomes and lysosomes
Research Areas
Citations (0)
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$ 365.00
$ 140.00
Cat# AP7391b
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