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COL4A1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 3
- PROTOCOLS
- BACKGROUND
Application 
| IF, WB, E |
|---|---|
| Primary Accession | P02462 |
| Other Accession | P02463 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 160611 Da |
| Antigen Region | 27-54 aa |
| Gene ID | 1282 |
|---|---|
| Other Names | Collagen alpha-1(IV) chain, Arresten, COL4A1 |
| Target/Specificity | This COL4A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-54 amino acids from the N-terminal region of human COL4A1. |
| Dilution | IF~~1:25 WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | COL4A1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | COL4A1 (HGNC:2202) |
|---|---|
| Function | Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. |
| Cellular Location | Secreted, extracellular space, extracellular matrix, basement membrane {ECO:0000250|UniProtKB:P02463} |
| Tissue Location | Highly expressed in placenta. |
Provided below are standard protocols that you may find useful for product applications.
Background
COL4A1 is the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter.
References
de Vries,L.S., Ann. Neurol. 65 (1), 12-18 (2009)
Mazouni,C., Br. J. Cancer 99 (1), 68-71 (2008)
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