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MGMT Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 2
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC, IHC-P, E |
|---|---|
| Primary Accession | P16455 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 21646 Da |
| Antigen Region | 156-182 aa |
| Gene ID | 4255 |
|---|---|
| Other Names | Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase, MGMT |
| Target/Specificity | This MGMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-182 amino acids from the C-terminal region of human MGMT. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MGMT Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MGMT |
|---|---|
| Function | Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. |
| Cellular Location | Nucleus. |
Provided below are standard protocols that you may find useful for product applications.
Background
MGMT is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. This protein repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the protein is irreversibly inactivated.
References
Kim,J.I., Suh,J.T. Hum. Pathol. 40 (7), 934-941 (2009)
Chen,S.P., Chiu,S.C. Genet Test Mol Biomarkers 13 (1), 67-71 (2009)
Slupphaug,G., Lettrem,I. Carcinogenesis 13 (10), 1769-1773 (1992)
Lee,S.M., Crowther,D. Br. J. Cancer 66 (2), 331-336 (1992)
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