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POLE3 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC, E |
|---|---|
| Primary Accession | Q9NRF9 |
| Other Accession | Q642A5, Q9JKP7, Q3SZN5 |
| Reactivity | Human |
| Predicted | Bovine, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 16860 Da |
| Antigen Region | 5-34 aa |
| Gene ID | 54107 |
|---|---|
| Other Names | DNA polymerase epsilon subunit 3, Arsenic-transactivated protein, AsTP, Chromatin accessibility complex 17 kDa protein, CHRAC-17, HuCHRAC17, DNA polymerase II subunit 3, DNA polymerase epsilon subunit p17, POLE3, CHRAC17 |
| Target/Specificity | This POLE3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-34 amino acids from the N-terminal region of human POLE3. |
| Dilution | WB~~1:1000 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | POLE3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | POLE3 |
|---|---|
| Synonyms | CHRAC17 |
| Function | Accessory component of the DNA polymerase epsilon complex (PubMed:10801849). Participates in DNA repair and in chromosomal DNA replication (By similarity). Forms a complex with CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1 (PubMed:10801849). Does not enhance nucleosome sliding activity of the ACF-5 ISWI chromatin remodeling complex (PubMed:14759371). |
| Cellular Location | Nucleus. |
| Tissue Location | Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.
References
Bolognese,F., Imbriano,C. Nucleic Acids Res. 28 (19), 3830-3838 (2000)
Li,Y., Pursell,Z.F. J. Biol. Chem. 275 (30), 23247-23252 (2000)
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