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JLP (SPAG9) Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P-Leica, WB, E |
|---|---|
| Primary Accession | O60271 |
| Other Accession | Q58A65 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 146205 Da |
| Antigen Region | 91-120 aa |
| Gene ID | 9043 |
|---|---|
| Other Names | C-Jun-amino-terminal kinase-interacting protein 4, JIP-4, JNK-interacting protein 4, Cancer/testis antigen 89, CT89, Human lung cancer oncogene 6 protein, HLC-6, JNK-associated leucine-zipper protein, JLP, Mitogen-activated protein kinase 8-interacting protein 4, Proliferation-inducing protein 6, Protein highly expressed in testis, PHET, Sperm surface protein, Sperm-associated antigen 9, Sperm-specific protein, Sunday driver 1, SPAG9, HSS, KIAA0516, MAPK8IP4, SYD1 |
| Target/Specificity | This JLP (SPAG9) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-120 amino acids from the N-terminal region of human JLP (SPAG9). |
| Dilution | WB~~1:2000 IHC-P-Leica~~1:250 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | JLP (SPAG9) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SPAG9 (HGNC:14524) |
|---|---|
| Function | The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module (PubMed:14743216). Regulates lysosomal positioning by acting as an adapter protein which links PIP4P1-positive lysosomes to the dynein- dynactin complex (PubMed:29146937). Assists PIKFYVE selective functionality in microtubule-based endosome-to-TGN trafficking (By similarity). |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:Q58A65}. Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:Q58A65}. Lysosome membrane. Note=Perinuclear distribution in response to stress signals such as UV radiation {ECO:0000250|UniProtKB:Q58A65} |
| Tissue Location | Expressed only in testis on the round spermatids of stage I, II and II. Absent in spermatogonia and spermatocyte [Isoform 3]: Expressed in testis. |
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Provided below are standard protocols that you may find useful for product applications.
Background
SPAG9, which is abundantly expressed in testicular haploid germ cells, is recognized by sperm-agglutinating antibodies and implicated in infertility.
References
Rana,R., Hum. Reprod. 21 (11), 2894-2900 (2006)
Rana,R., Biochem. Biophys. Res. Commun. 340 (1), 158-164 (2006)
Jagadish,N., Keio J Med 54 (2), 66-71 (2005)
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