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PHB2 Antibody (Y248)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P-Leica, E |
|---|---|
| Primary Accession | Q99623 |
| Other Accession | Q5XIH7, O35129, Q2HJ97, NP_009204 |
| Reactivity | Human, Mouse, Rat |
| Predicted | Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 33296 Da |
| Antigen Region | 225-255 aa |
| Gene ID | 11331 |
|---|---|
| Other Names | Prohibitin-2, B-cell receptor-associated protein BAP37, D-prohibitin, Repressor of estrogen receptor activity, PHB2 {ECO:0000312|EMBL:AAH147661, ECO:0000312|HGNC:HGNC:30306} |
| Target/Specificity | This PHB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-255 amino acids from human PHB2. |
| Dilution | WB~~1:1000 IHC-P-Leica~~1:250 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PHB2 Antibody (Y248) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PHB2 {ECO:0000312|EMBL:AAH14766.1, ECO:0000312|HGNC:HGNC:30306} |
|---|---|
| Function | Protein with pleiotropic attributes mediated in a cell- compartment- and tissue-specific manner, which include the plasma membrane-associated cell signaling functions, mitochondrial chaperone, and transcriptional co-regulator of transcription factors and sex steroid hormones in the nucleus. |
| Cellular Location | Mitochondrion inner membrane. Cytoplasm. Nucleus. Cell membrane Note=Localizes within both nucleus and cytoplasm in proliferative primary myoblasts and mostly within the nucleus of differentiated primary myoblasts. [Isoform 2]: Mitochondrion inner membrane |
| Tissue Location | Expressed in myoblasts. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
PHB2 acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. It functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity and is probably involved in regulating mitochondrial respiration activity and in aging.
References
Takata,H., Curr. Biol. 17 (15), 1356-1361 (2007)
Kasashima,K., J. Biol. Chem. 281 (47), 36401-36410 (2006)
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