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DAPK2 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - DAPK2 Antibody (N-term) AP7218A
    The anti-DAPK2 Pab (Cat. #AP7218a) is used in Western blot to detect DAPK2 in mouse lung tissue lysate.
    detail
  • WB - DAPK2 Antibody (N-term) AP7218A
    Western blot analysis of DAPK2(arrow) using rabbit polyclonal DAPK2 Antibody (N-term) (Cat. #AP7218a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DAPK2 gene (Lane 2) (Origene Technologies).
    detail
  • IHC-P - DAPK2 Antibody (N-term) AP7218A
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
    detail
  • IHC-P - DAPK2 Antibody (N-term) AP7218A
    Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with DAPK2 Antibody (N-term V55) (Cat.#AP7218a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q9UIK4
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 42898 Da
Antigen Region 40-70 aa
Additional Information
Gene ID 23604
Other Names Death-associated protein kinase 2, DAP kinase 2, DAP-kinase-related protein 1, DRP-1, DAPK2
Target/Specificity This DAPK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-70 amino acids from the N-terminal region of human DAPK2.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDAPK2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DAPK2
Function Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell death signals, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation (PubMed:17347302). Regulates granulocytic motility by controlling cell spreading and polarization (PubMed:24163421).
Cellular Location Cytoplasm. Cytoplasmic vesicle, autophagosome lumen
Tissue Location Expressed in neutrophils and eosinophils (PubMed:24163421). Isoform 2 is expressed in embryonic stem cells (at protein level). Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle
Research Areas
Citations (0)
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Background

DAPK2 belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites.

References

Satoh, A., et al., Br. J. Cancer 86(11):1817-1823 (2002).
Chan, M.W., et al., Clin. Cancer Res. 8(2):464-470 (2002).
Wong, T.S., et al., Clin. Cancer Res. 8(2):433-437 (2002).
Shani, G., et al., EMBO J. 20(5):1099-1113 (2001).
Inbal, B., et al., Mol. Cell. Biol. 20(3):1044-1054 (2000).

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$ 365.00
$ 140.00
Cat# AP7218A
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