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LYK5 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC, IF, E |
|---|---|
| Primary Accession | Q7RTN6 |
| Other Accession | Q3UUJ4 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 48369 Da |
| Antigen Region | 12-41 aa |
| Gene ID | 92335 |
|---|---|
| Other Names | STE20-related kinase adapter protein alpha, STRAD alpha, STE20-related adapter protein, Serologically defined breast cancer antigen NY-BR-96, STRADA, LYK5 {ECO:0000312|EMBL:AAP422801}, STRAD |
| Target/Specificity | This LYK5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-41 amino acids from the N-terminal region of human LYK5. |
| Dilution | IF~~1:25 WB~~1:2000 FC~~1:25 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | LYK5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | STRADA |
|---|---|
| Synonyms | LYK5 {ECO:0000312|EMBL:AAP42280.1}, STRA |
| Function | Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation. |
| Cellular Location | Nucleus. Cytoplasm |
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Provided below are standard protocols that you may find useful for product applications.
Background
LYK5, also STE20-related Adaptor Protein (STRAD) are members of STE-20 like kinase family that are known to stimulate MAPK pathways by directly activating MAPKKK. LYK5 is a novel pseudokinase member of this family consisting of a STE-20 like kinase domain but lacks several residues that are required for its catalytic activity. It specifically binds LKB1 and plays a key role in regulating tumor suppressor activities of LKB1. It functions as an upstream activator of LKB1 and also directs the sub-cellular localization of LKB1 by anchoring it in the cytoplasm. STRAD-LKB1 interaction results in phosphorylation of STRAD and enhanced autophosphorylation of LKB1.
References
Baas, A.F., et al., EMBO J. 22(12):3062-3072 (2003).
Scanlan, M.J., et al., Cancer Immun. 1, 4 (2001).
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