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CAMKK2 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 3
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q96RR4 |
| Other Accession | O88831, Q8C078 |
| Reactivity | Human |
| Predicted | Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 64746 Da |
| Antigen Region | 483-512 aa |
| Gene ID | 10645 |
|---|---|
| Other Names | Calcium/calmodulin-dependent protein kinase kinase 2, CaM-KK 2, CaM-kinase kinase 2, CaMKK 2, Calcium/calmodulin-dependent protein kinase kinase beta, CaM-KK beta, CaM-kinase kinase beta, CaMKK beta, CAMKK2, CAMKKB, KIAA0787 |
| Target/Specificity | This CAMKK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 483-512 amino acids from the C-terminal region of human CAMKK2. |
| Dilution | WB~~1:1000 IHC-P~~1:50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CAMKK2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CAMKK2 |
|---|---|
| Synonyms | CAMKKB, KIAA0787 |
| Function | Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Efficiently phosphorylates 5'-AMP-activated protein kinase (AMPK) trimer, including that consisting of PRKAA1, PRKAB1 and PRKAG1. This phosphorylation is stimulated in response to Ca(2+) signals (By similarity). Seems to be involved in hippocampal activation of CREB1 (By similarity). May play a role in neurite growth. Isoform 3 may promote neurite elongation, while isoform 1 may promoter neurite branching. |
| Cellular Location | Nucleus. Cytoplasm. Cell projection, neuron projection. Note=Predominantly nuclear in unstimulated cells, relocalizes into cytoplasm and neurites after forskolin induction. |
| Tissue Location | Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung |
Provided below are standard protocols that you may find useful for product applications.
Background
CAMKK2 belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. CAMKK2 appears to be involved in hippocampal activation of CREB1.
References
Hsu, L.S., et al., J. Biol. Chem. 276(33):31113-31123 (2001).
Hsu, L.S., et al., J. Biomed. Sci. 5(2):141-149 (1998).
Anderson, K.A., et al., J. Biol. Chem. 273(48):31880-31889 (1998).
Ishikawa, Y., et al., FEBS Lett. 550 (1-3), 57-63 (2000).
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