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CKB Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CKB Antibody (N-term) AP7059a
    The anti-CKB Pab (Cat. #AP7059a) is used in Western blot to detect CKB in mouse colon tissue lysate (Lane 1) and Y79 cell lysate (Lane 2).
    detail
  • WB - CKB Antibody (N-term) AP7059a
    Western blot analysis of CKB (arrow) using rabbit polyclonal CKB Antibody (N5) (Cat. #AP7059a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CKB gene.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P12277
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 42644 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 1152
Other Names Creatine kinase B-type, B-CK, Creatine kinase B chain, CKB, CKBB
Target/Specificity This CKB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CKB.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCKB Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CKB (HGNC:1991)
Synonyms CKBB
Function Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate) (PubMed:8186255). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation (By similarity). During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N- phosphocreatine as heat without performing any mechanical or chemical work (By similarity).
Cellular Location Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q04447}. Mitochondrion {ECO:0000250|UniProtKB:Q04447}. Cell membrane. Note=Localizes to the mitochondria of thermogenic fat cells via the internal MTS-like signal (iMTS-L) region {ECO:0000250|UniProtKB:Q04447}
Research Areas
Citations (0)
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Background

Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. The CKB isoform, present in many tissues but especially brain, is a cytoplasmic enzyme involved in energy homeostasis. CKB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. Creatine kinase B-driven energy transfer in the brain is important for habituation and spatial learning behaviour, mossy fibre field size and determination of seizure susceptibility. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.

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$ 365.00
$ 140.00
Cat# AP7059a
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