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DAPK2 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 2
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q9UIK4 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 42898 Da |
| Antigen Region | 1-30 aa |
| Gene ID | 23604 |
|---|---|
| Other Names | Death-associated protein kinase 2, DAP kinase 2, DAP-kinase-related protein 1, DRP-1, DAPK2 |
| Target/Specificity | This DAPK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human DAPK2. |
| Dilution | WB~~1:2000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | DAPK2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DAPK2 |
|---|---|
| Function | Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell death signals, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation (PubMed:17347302). Regulates granulocytic motility by controlling cell spreading and polarization (PubMed:24163421). |
| Cellular Location | Cytoplasm. Cytoplasmic vesicle, autophagosome lumen |
| Tissue Location | Expressed in neutrophils and eosinophils (PubMed:24163421). Isoform 2 is expressed in embryonic stem cells (at protein level). Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle |
Provided below are standard protocols that you may find useful for product applications.
Background
DAPK2 belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites.
References
Satoh, A., et al., Br. J. Cancer 86(11):1817-1823 (2002).
Chan, M.W., et al., Clin. Cancer Res. 8(2):464-470 (2002).
Wong, T.S., et al., Clin. Cancer Res. 8(2):433-437 (2002).
Shani, G., et al., EMBO J. 20(5):1099-1113 (2001).
Inbal, B., et al., Mol. Cell. Biol. 20(3):1044-1054 (2000).
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