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ADRBK1 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P25098 |
| Other Accession | P26817, Q99MK8, P21146 |
| Reactivity | Human |
| Predicted | Bovine, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 79574 Da |
| Antigen Region | 633-660 aa |
| Gene ID | 156 |
|---|---|
| Other Names | Beta-adrenergic receptor kinase 1, Beta-ARK-1, G-protein coupled receptor kinase 2, ADRBK1, BARK, BARK1, GRK2 |
| Target/Specificity | This ADRBK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 633-660 amino acids from the C-terminal region of human ADRBK1. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ADRBK1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GRK2 (HGNC:289) |
|---|---|
| Synonyms | ADRBK1, BARK, BARK1 |
| Function | Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them (PubMed:19715378). Key regulator of LPAR1 signaling (PubMed:19306925). Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor (PubMed:19306925). Desensitizes LPAR1 and LPAR2 in a phosphorylation- independent manner (PubMed:19306925). Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by facilitating the trafficking of SMO into the cilium and the stimulation of SMO activity (By similarity). Inhibits relaxation of airway smooth muscle in response to blue light (PubMed:30284927). |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:P26817}. Cell membrane {ECO:0000250|UniProtKB:P21146}. Postsynapse {ECO:0000250|UniProtKB:P26817}. Presynapse {ECO:0000250|UniProtKB:P26817} |
| Tissue Location | Expressed in peripheral blood leukocytes. |
Provided below are standard protocols that you may find useful for product applications.
Background
Beta-adrenergic receptor kinase (ADRBK1), also known as GRK2, phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. ADRBK1 is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Heart failure is accompanied by severely impaired beta-adrenergic receptor (beta-AR) function. An important mechanism for the rapid desensitization of beta-AR function is agonist-stimulated receptor phosphorylation by the beta-AR kinase (beta-ARK1), an enzyme known to be elevated in failing human heart tissue. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart.
References
Li, J., et al., J. Biol. Chem. 278(32):30219-30226 (2003).
Wan, K.F., et al., J. Biol. Chem. 278(20):18658-18663 (2003).
Yang, X.L., et al., World J. Gastroenterol. 9(4):800-803 (2003).
Hagen, S.A., et al., Anesthesiology 98(2):343-348 (2003).
Eichmann, T., et al., J. Biol. Chem. 278(10):8052-8057 (2003).
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