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> home > Products > Primary Antibodies > Antibody Collections > Immune system process > CD28 Antibody (C-term)
CD28 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IF, WB, E |
|---|---|
| Primary Accession | P10747 |
| Other Accession | P42069 |
| Reactivity | Human |
| Predicted | Rabbit |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 25066 Da |
| Antigen Region | 182-208 aa |
| Gene ID | 940 |
|---|---|
| Other Names | T-cell-specific surface glycoprotein CD28, TP44, CD28, CD28 |
| Target/Specificity | This CD28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-208 amino acids from the C-terminal region of human CD28. |
| Dilution | IF~~1:10~50 WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CD28 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CD28 |
|---|---|
| Function | Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis (PubMed:1650475, PubMed:7568038). Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells (PubMed:24665965). Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets (PubMed:1650475, PubMed:35397202). Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways (PubMed:21964608, PubMed:24665965, PubMed:7568038). In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells (PubMed:8617933). |
| Cellular Location | Cell membrane; Single-pass type I membrane protein |
| Tissue Location | Expressed in T-cells and plasma cells, but not in less mature B-cells |
Research Areas
Citations (0)
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info@abcepta.com, and receive a free "I Love Antibodies" mug.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
CD28 costimulation is essential for CD4-positive T-cell proliferation, survival, interleukin-2 production, and T-helper type-2 (Th2) development.
References
Zocchi,M.R., et.al., Eur. J. Cancer 28A (4-5), 749-754 (1992)
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$ 182.50
$ 70.00
Cat# AP6982B
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