CD28 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IF, WB, E |
---|---|
Primary Accession | P10747 |
Other Accession | P42069 |
Reactivity | Human |
Predicted | Rabbit |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 25066 Da |
Antigen Region | 182-208 aa |
Gene ID | 940 |
---|---|
Other Names | T-cell-specific surface glycoprotein CD28, TP44, CD28, CD28 |
Target/Specificity | This CD28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-208 amino acids from the C-terminal region of human CD28. |
Dilution | IF~~1:10~50 WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | CD28 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CD28 |
---|---|
Function | Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037). |
Cellular Location | Membrane; Single-pass type I membrane protein. |
Tissue Location | Expressed in T-cells and plasma cells, but not in less mature B-cells |
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Provided below are standard protocols that you may find useful for product applications.
Background
CD28 costimulation is essential for CD4-positive T-cell proliferation, survival, interleukin-2 production, and T-helper type-2 (Th2) development.
References
Zocchi,M.R., et.al., Eur. J. Cancer 28A (4-5), 749-754 (1992)
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