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LIF Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - LIF Antibody (Center) AP6981C
    All lanes : Anti- LIF Antibody (Center) at 1:1000 dilution Lane 1: KG-1 whole cell lysate Lane 2:Human spleen whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - LIF Antibody (Center) AP6981C
    Anti-LIF Antibody (Center) at 1:1000 dilution + Rat spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P-Leica - LIF Antibody (Center) AP6981C
    Immunohistochemical analysis of paraffin-embedded human kidney tissue using AP6981C performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • FC - LIF Antibody (Center) AP6981C
    Overlay histogram showing U-2 OS cells stained with AP6981C(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP6981C, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P-Leica, E
Primary Accession P15018
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 22008 Da
Antigen Region 72-101 aa
Additional Information
Gene ID 3976
Other Names Leukemia inhibitory factor, LIF, Differentiation-stimulating factor, D factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin, LIF, HILDA
Target/Specificity This LIF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-101 amino acids from the Central region of human LIF.
Dilution WB~~1:1000
IHC-P-Leica~~1:500
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLIF Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LIF
Synonyms HILDA
Function LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Cellular Location Secreted.
Research Areas
Citations (0)
citation

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Background

LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.

References

Novotny,Z., et.al., Folia Biol. (Praha) 55 (3), 92-97 (2009)

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$ 182.50
$ 70.00
Cat# AP6981C
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