DIO1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | P49895 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 28924 Da |
Antigen Region | 30-57 aa |
Gene ID | 1733 |
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Other Names | Type I iodothyronine deiodinase, 5DI, DIOI, Type 1 DI, Type-I 5'-deiodinase, DIO1, ITDI1, TXDI1 |
Target/Specificity | This DIO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-57 amino acids from the N-terminal region of human DIO1. |
Dilution | WB~~1:2000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | DIO1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | DIO1 |
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Synonyms | ITDI1, TXDI1 |
Function | Responsible for the deiodination of T4 (3,5,3',5'- tetraiodothyronine) into T3 (3,5,3'-triiodothyronine) and of T3 into T2 (3,3'-diiodothyronine). Plays a role in providing a source of plasma T3 by deiodination of T4 in peripheral tissues such as liver and kidney. |
Cellular Location | Endoplasmic reticulum membrane; Single-pass membrane protein |
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Provided below are standard protocols that you may find useful for product applications.
Background
DIO1 is a thiol-requiring propylthiouracil-sensitive oxidoreductase. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). It also degrades both hormones by inner ring deiodination (IRD).
References
Landa,I., et.al., PLoS Genet. 5 (9), E1000637 (2009)
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